Evaluating drained water quality in a pilot worm-sludge treatment reed bed planted with Arundo donnas in the Mediterranean climate.

This study evaluated the impact of incorporating earthworms (Eisenia fetida) on the drained water quality from a sludge treatment reed bed. The experiment encompassed four setups of treatment beds in two replicates: planted with Arundo donax and addition of earthworms, planted without earthworms, unplanted with earthworms, and treatment bed without plants nor earthworms as control. The units were fed every two weeks with mixed sewage sludge, a blend of primary and secondary sludge over 24 cycles. The mixed sewage sludge had mean dry and volatile solid contents of 24.71 g.DS.L-1 (± 13.67) and 19.14 g.VS.L-1 (± 10.29) resulting a sludge loading rate of 43.59 kg.DS.m-2.year-1 (± 14.49). The inclusion of earthworms in the planted unit reduced release masses of total suspended solids, chemical oxygen demand, nitrate and phosphorous by 43, 45, 75 and 45 % compared to the planted unit. Plant biomass production increased by 43 % with the earthworm presence. The removal efficiency of the units improved after a ramp-up phase (after six months feeding) of which the concentration of TSS, COD and Escherichia coli met limits for water reuse while nitrogen components and phosphorous surpassed the limits. The planted unit with earthworms removed 99 and 99 % of TSS and COD, respectively. Overall, water loss namely through evapotranspiration and earthworm hydration need, positively correlated with pollutant concentration, and earthworm-planted unit had 46 % higher water loss compared to control unit.

Synthesis of a novel functionalized biosorbent from mango stone and its application in the pharmaceutical's removal from water and a synthetic mixture.

This paper explores the feasibility of functionalizing mango stones with iron oxide magnetic nanoparticles (MS-Fe3O4) by coprecipitation in batch adsorption processes. The synthesized material was characterized and applied in chloroquine (CQN) and sertraline hydrochloride (SER) removal from contaminated waters. The biosorbent was subjected to a regenerative study and treatment using a synthetic mixture of contaminants to evaluate its applicability in real effluents. The biosorbent was analyzed by transmission electron microscopy images, scanning electron microscopy, dispersive X-ray spectroscopy, Fourier transform infrared spectra, and zeta potential to characterize its chemical and morphology properties. The techniques applied showed the effectiveness of the proposed modification. In the adsorption experiments, the optimal adsorbent dosage was 0.01 g for both contaminants. The pH strongly influenced the adsorption of the drugs on MS-Fe3O4, and the best results were obtained in the pH range of 5-6. Kinetic data showed a better fit to the pseudo-second-order model, and the equilibrium time was achieved in 16 h for CQN and 4 h for SER. Isotherm studies revealed maximum adsorptive capacities of 49.42 and 64.79 mg g-1, respectively, for CQN and SER, at 318 K, demonstrating that the increase in temperature is a favorable factor, and the Sips model better describes the process. The thermodynamic parameters indicate an endothermic (ΔH° >0), spontaneous (ΔG° <0), and reversible (ΔS° >0) nature of the adsorption. This process is essentially governed by physical forces, such as hydrogen and π-π bonds. However, it is also valid to consider the presence of electrostatic forces due to the ionizing nature of CQN and SER. The MS-Fe3O4 biosorbent showed good performance when evaluated in a synthetic mixture of four contaminants, with an overall removal efficiency of approximately 86% and the regenerative capacity of three reusing cycles.

Water balance analysis in a novel pilot-scale of the Worm-sludge treatment reed bed (W-STRB) planted with Arundo donax.

A one-year study of sludge treatment reed bed assisted with earthworms (W-STRB) was conducted in a temperate climate. The effects of using Eisenia fetid and Arundo donax on W-STRB water balance (WB) and dewatering efficiency (DE) were investigated. Four different bed configurations were tested: worm-planted (WP), planted (P), worm-control (W), and control (C), duplicated resulting in a total of eight units. The beds received a total of 24 cycles of mixed sewage sludge twice per month (average loading rate: 43.59 kg.DS. m-2.year-1). It was found seasonal variation played a significant role in WB and DE. During the dry season, the thickness of the residual sludge (RS) layer was less than 1 cm, with a dry solid (DS) content of over 80%, in contrast, the wet season indicated an increase in RS thickness to nearly 30 cm (DS < 15 % for all units). The WP unit exhibited the lowest RS accumulation, 22% less than the P, W, and C units. The subsurface layer had a 5% lower volatile solids (VS) content compared to the surface layers. After 132 days of a final resting, WP unit had the highest RS volume reduction of 65 % (DS = 71 % and VS = 53 %) and a RS thickness of 6 cm indicating a 10 % higher stabilization compared to P unit. The population of earthworms was 30% higher in the WP unit compared to the W unit. As the subsurface DS exceeded 20 % during the dry season, the population increased. The WP unit showed a 43% higher above-ground plant biomass compared to the P unit. In WB analysis, evapotranspiration (ET) was 46% higher in the WP unit (average daily ET = 5.44 mm in the dry season). The main process of water loss was through drainage and Awhile water content in RS layer was 57 % during feeding period. The water percolation rate of all units decreased by 99%, particularly during the wet season, reaching less than 0.1 m.day-1.

Mango Peel Nanofiltration Concentrates to Enhance Anaerobic Digestion of Slurry from Piglets Fed with Laminaria.

The environmental impact of biowaste generated during animal production can be mitigated by applying a circular economy model: recycling, reinventing the life cycle of biowaste, and developing it for a new use. The aim of this study was to evaluate the effect of adding sugar concentrate solutions obtained from the nanofiltration of fruit biowaste (mango peel) to slurry from piglets fed with diets incorporating macroalgae on biogas production performance. The nanofiltration of ultrafiltration permeates from aqueous extracts of mango peel was carried out using membranes with a molecular weight cut-off of 130 Da until a volume concentration factor of 2.0 was reached. A slurry resulting from piglets fed with an alternative diet with the incorporation of 10% Laminaria was used as a substrate. Three different trials were performed sequentially: (i) a control trial (AD0) with faeces resulting from a cereal and soybean-meal-based diet (S0); (ii) a trial with S1 (10% L. digitata) (AD1), and (iii) an AcoD trial to assess the effect of the addition of a co-substrate (20%) to S1 (80%). The trials were performed in a continuous-stirred tank reactor (CSTR) under mesophilic conditions (37.0 ± 0.4 °C), with a hydraulic retention time (HRT) of 13 days. The specific methane production (SMP) increased by 29% during the anaerobic co-digestion process. These results can support the design of alternative valorisation routes for these biowastes, contributing to sustainable development goals.

Evaluation of hydrochar from peach stones for caffeine removal from aqueous medium and treatment of a synthetic mixture.

The presence of micropollutants, such as caffeine (CAF), has been detected throughout the world, since conventional treatment plants are not able to properly degrade them. CAF is a widely consumed stimulant, and has been demanding the development of efficient methodologies for its removal. Aiming at the agriculture waste valorization, a new hydrochar was developed based on chemical and thermal modification of peach stones (mod-PS) for CAF removal from water and from a synthetic mixture. The morphology, functional groups and surface electrical charge of the adsorbent were characterized by SEM, FTIR and zeta potential, respectively. Regarding CAF adsorption performance, the equilibrium time was reached at 480 min and the pseudo-second-order model presented the best fit for the experimental data. The maximum adsorption capacity was 68.39 mg g-1 (298 K) and the Langmuir model exhibited a better fit for the isothermal data. The thermodynamic properties confirmed that the process was exothermic, spontaneous and reversible. The main adsorption mechanisms were hydrogen bonds and π-interactions. The global removal efficiency was satisfactory in the synthetic mixture simulating real wastewater (67%). Therefore, the proposed new hydrochar has potential application as a low-cost adsorbent for CAF removal.

Moringa as a household water purification method - community perception and pilot study in Guinea-Bissau.

BACKGROUND: Public perceptions of water-related issues are still under-researched topics. The current paper intends to explore a local community's perceptions regarding household water purification (HWP) strategies, namely before and after trying a new method: moringa seeds powder (moringa-teabag). METHODS: In September 2020, six focus group discussions (N = 65) assessing perceptions about the usefulness of Moringa oleifera Lam (Moringaceae) as a HWP method (before moringa-based HWP trials), and questionnaires (N = 104) evaluating successes and identifying difficulties (after one week of moringa-based HWP trials). Participants were all women aged over 18 years, living in Ondame, Biombo region, Guinea-Bissau. Data were analyzed using qualitative and quantitative approaches. RESULTS: The focus group discussions revealed that people are aware of the fact that water can transmit diseases. Although certain persons showed concern about shallow well water safety, people generally underestimate the risk, as they trust tubewell water. Not everyone had an understanding of what water contamination is, or the concept of medical importance. Some respondents declared they use traditional methods such as boiling and bleach to treat water before drinking. However, those who reported no kind of treatment indicated reasons such as lack of time, cost, and bleach's taste and smell. In the questionnaire, more than half of the participants (68%) reported treating water before consumption. Nevertheless, these results are not consistent with our field notes. Participants demonstrated a strong belief in the capacity of moringa-teabags to purify water and even consider them better or much better (81%) than other methods. Participants asked for more information on moringa-teabag for household water purification. CONCLUSION: More information on water treatment and water safety would help to raise public awareness about waterborne diseases. These findings could be used to promote greater adherence to moringa-based HWP as an alternative to household water treatment.

Phylogenomics and gene selection in Aspergillus welwitschiae: Possible implications in the pathogenicity in Agave sisalana.

Aspergillus welwitschiae causes bole rot disease in sisal (Agave sisalana and related species) which affects the production of natural fibers in Brazil, the main worldwide producer of sisal fibers. This fungus is a saprotroph with a broad host range. Previous research established A. welwitschiae as the only causative agent of bole rot in the field, but little is known about the evolution of this species and its strains. In this work, we performed a comparative genomics analysis of 40 Aspergillus strains. We show the conflicting molecular identity of this species, with one sisal-infecting strain sharing its last common ancestor with Aspergillus niger, having diverged only 833 thousand years ago. Furthermore, our analysis of positive selection reveals sites under selection in genes coding for siderophore transporters, Sodium­calcium exchangers, and Phosphatidylethanolamine-binding proteins (PEBPs). Herein, we discuss the possible impacts of these gene functions on the pathogenicity in sisal.

Membrane Technology for Valorization of Mango Peel Extracts.

Mango peel is rich in nutritional and functional compounds, such as carbohydrates, dietary fibers, proteins, and phenolic compounds, with high potential to be applied in the food industry. Most of the investigation about recovery of bioactive compounds from fruit bioproducts involves extraction techniques and further separation of target compounds. There is still a lack of information about the potential of membrane processes to recover the nutritive/functional compounds present in aqueous extracts of those bioproducts. This research is addressed to study the performance of ultrafiltration (UF), followed by nanofiltration (NF) of UF permeates, to fractionate the compounds present in aqueous extracts of mango peel. Both UF and NF concentration processes were carried up to a volume concentration factor of 2.0. Membranes with molecular weight cut-offs of 25 kDa and 130 Da were used in the UF and NF steps, respectively. UF and NF concentrates showed antioxidant activity, attributed to the presence of phenolic compounds, with rejections of about 75% and 98.8%, respectively. UF membranes totally rejected the higher molecular weight compounds, and NF membranes almost totally concentrated the fermentable monosaccharides and disaccharides. Therefore, it is envisaged that NF concentrates can be utilized by the food industry or for bioenergy production.

Comparative Effect of Three Different Exercise Intensities in Combination with Diazoxide on Contraction Capacity and Oxidative Stress of Skeletal Muscle in Obese Rats.

Obesity is a chronic disease that impairs skeletal muscle function, affects the ability to contract, and promotes the development of fatigue. For this reason, the study of treatments that seek to reduce the harmful effects of obesity on muscle tissue has been deepened. Diazoxide treatment and various exercise protocols have been proposed to protect skeletal muscle against oxidative stress and its effects. However, the intensity and duration of exercise combined with diazoxide that would obtain the best results for improving skeletal muscle function in obese rats is unknown. To this end, this study evaluated the effects of three different exercise intensities combined with diazoxide on contraction capacity, resistance to fatigue, markers of oxidative stress, lipid peroxidation, ROS, and glutathione redox status of skeletal muscle. The results showed that treatments with diazoxide and exercise at different intensities improved muscle contraction capacity by reducing oxidative stress during obesity, with the best results being obtained with low-intensity exercise in combination with diazoxide. Therefore, these results suggest that diazoxide and low-intensity exercise improve muscle function during obesity by decreasing oxidative stress with the same efficiency as a moderate-intensity exercise protocol.

Mulheres Médicas: Burnout durante a Pandemia de COVID-19 no Brasil / Women Physicians: Burnout during the COVID-19 Pandemic in Brazil

Resumo Fundamento A COVID-19 adicionou um fardo enorme sobre os médicos ao redor do mundo, especialmente as mulheres médicas, que são afetadas pelo aumento da carga de trabalho e pela perda da qualidade de vida. Objetivo Avaliar os efeitos da pandemia de COVID-19 na qualidade de vida, burnout e espiritualidade de médicas brasileiras que atendem pacientes com COVID-19 direta ou indiretamente. Método Estudo prospectivo, observacional realizado de 28 de julho a 27 de setembro de 2020, no Brasil, com mulheres médicas de 47 especialidades, a mais frequente sendo a cardiologia (22,8%), sem restrição de idade. Elas responderam voluntariamente um questionário online com questões sobre características demográficas e socioeconômicas, qualidade de vida (WHOQOL-brief) e espiritualidade (WHOQOL-SRPB) e enunciados do Oldenburg Burnout Inventory. A análise estatística utilizou o software R, regressão beta, árvores de classificação e matriz de correlação policórica, com nível de significância de 5%. Resultados Das 769 respondentes, 61,6% relataram sinais de burnout. Cerca de 64% relataram perda salarial de até 50% durante a pandemia. Algumas relataram falta de energia para as tarefas diárias, sentimentos negativos frequentes, insatisfação com a capacidade para o trabalho, e que cuidar de outras pessoas não agregava sentido às suas vidas. Os sentimentos negativos correlacionaram-se negativamente com a satisfação com a vida sexual, a satisfação com as relações pessoais e a energia para as tarefas diárias. A incapacidade de permanecer otimista em tempos de incerteza correlacionou-se positivamente com a sensação de insegurança no dia a dia e com o não reconhecimento de que cuidar de outras pessoas trouxesse sentido à vida. Conclusão O presente estudo mostrou uma alta frequência de burnout entre as médicas brasileiras que responderam ao questionário durante a pandemia de COVID-19. Apesar disso, apresentavam uma qualidade de vida relativamente boa e acreditavam que a espiritualidade trazia-lhes conforto e segurança nos momentos difíceis.

Abstract Background COVID-19 has placed a tremendous burden on physicians worldwide, especially women physicians, affected by increased workload and loss of quality of life. Objective To assess the effects of the COVID-19 pandemic on the quality of life, burnout and spirituality of Brazilian women physicians directly or indirectly providing care to COVID-19 patients. Methods Prospective, observational study performed from July 28 to September 27, 2020, in Brazil, with women physicians from 47 specialities, the most frequent being cardiology (22.8%), with no age restriction. They voluntarily answered an online survey with questions on demographic and socioeconomic characteristics, quality of life (WHOQOL-brief), spirituality (WHOQOL-SRPB), and statements from the Oldenburg Burnout Inventory. Statistical analysis used the R software, beta regression, classification trees, and polychoric correlation matrix, with a 5% of significance level. Results Of the 769 respondents, 61.6% reported signs of burnout. About 64% reported wage loss of up to 50% during the pandemic. Some reported lack of energy for daily tasks, frequent negative feelings, dissatisfaction with capability for work, and caring for others not adding meaning to their lives. Negative feelings correlated negatively with satisfaction with sexual life and personal relations, and energy for daily tasks. The inability to remain optimistic in times of uncertainty correlated positively with feeling unsafe daily and not acknowledging that caring for others brings meaning to life. Conclusion This study showed a high frequency of burnout among Brazilian women physicians who answered the survey during the COVID-19 pandemic. Nevertheless, they presented with a relatively good quality of life and believed that spirituality comforted and reassured them in hard times.

Women Physicians: Burnout during the COVID-19 Pandemic in Brazil. / Mulheres Médicas: Burnout durante a Pandemia de COVID-19 no Brasil.

BACKGROUND: COVID-19 has placed a tremendous burden on physicians worldwide, especially women physicians, affected by increased workload and loss of quality of life. OBJECTIVE: To assess the effects of the COVID-19 pandemic on the quality of life, burnout and spirituality of Brazilian women physicians directly or indirectly providing care to COVID-19 patients. METHODS: Prospective, observational study performed from July 28 to September 27, 2020, in Brazil, with women physicians from 47 specialities, the most frequent being cardiology (22.8%), with no age restriction. They voluntarily answered an online survey with questions on demographic and socioeconomic characteristics, quality of life (WHOQOL-brief), spirituality (WHOQOL-SRPB), and statements from the Oldenburg Burnout Inventory. Statistical analysis used the R software, beta regression, classification trees, and polychoric correlation matrix, with a 5% of significance level. RESULTS: Of the 769 respondents, 61.6% reported signs of burnout. About 64% reported wage loss of up to 50% during the pandemic. Some reported lack of energy for daily tasks, frequent negative feelings, dissatisfaction with capability for work, and caring for others not adding meaning to their lives. Negative feelings correlated negatively with satisfaction with sexual life and personal relations, and energy for daily tasks. The inability to remain optimistic in times of uncertainty correlated positively with feeling unsafe daily and not acknowledging that caring for others brings meaning to life. CONCLUSION: This study showed a high frequency of burnout among Brazilian women physicians who answered the survey during the COVID-19 pandemic. Nevertheless, they presented with a relatively good quality of life and believed that spirituality comforted and reassured them in hard times.

FUNDAMENTO: A COVID-19 adicionou um fardo enorme sobre os médicos ao redor do mundo, especialmente as mulheres médicas, que são afetadas pelo aumento da carga de trabalho e pela perda da qualidade de vida. OBJETIVO: Avaliar os efeitos da pandemia de COVID-19 na qualidade de vida, burnout e espiritualidade de médicas brasileiras que atendem pacientes com COVID-19 direta ou indiretamente. MÉTODO: Estudo prospectivo, observacional realizado de 28 de julho a 27 de setembro de 2020, no Brasil, com mulheres médicas de 47 especialidades, a mais frequente sendo a cardiologia (22,8%), sem restrição de idade. Elas responderam voluntariamente um questionário online com questões sobre características demográficas e socioeconômicas, qualidade de vida (WHOQOL-brief) e espiritualidade (WHOQOL-SRPB) e enunciados do Oldenburg Burnout Inventory. A análise estatística utilizou o software R, regressão beta, árvores de classificação e matriz de correlação policórica, com nível de significância de 5%. RESULTADOS: Das 769 respondentes, 61,6% relataram sinais de burnout. Cerca de 64% relataram perda salarial de até 50% durante a pandemia. Algumas relataram falta de energia para as tarefas diárias, sentimentos negativos frequentes, insatisfação com a capacidade para o trabalho, e que cuidar de outras pessoas não agregava sentido às suas vidas. Os sentimentos negativos correlacionaram-se negativamente com a satisfação com a vida sexual, a satisfação com as relações pessoais e a energia para as tarefas diárias. A incapacidade de permanecer otimista em tempos de incerteza correlacionou-se positivamente com a sensação de insegurança no dia a dia e com o não reconhecimento de que cuidar de outras pessoas trouxesse sentido à vida. CONCLUSÃO: O presente estudo mostrou uma alta frequência de burnout entre as médicas brasileiras que responderam ao questionário durante a pandemia de COVID-19. Apesar disso, apresentavam uma qualidade de vida relativamente boa e acreditavam que a espiritualidade trazia-lhes conforto e segurança nos momentos difíceis.

Sludge Treatment Reed Bed under different climates: A review using meta-analysis.

Sludge Treatment Reed Beds (STRBs) have been used worldwide over the past few decades. This review aims to overarchingly identify and appraise the currently available knowledge of STRB technology and discern climatic patterns through Meta-Analysis (MA). We systematically searched Google Scholar, Scopus, and Web of Science databases (up to Dec 2021) via a combination of keywords to identify English-language studies published in peer-reviewed journals. Of 142 potential articles, 73 studies met the present review objectives and inclusion criteria. Four STRB classifications including typical STRB, earthworm STRB, Sludge Treatment Electro Wetland (STEW), and earthworm STEW were found since 1990. The data and information on STRBs' configuration, operational parameters in terms of location, type of sewage sludge, study scale, Sludge Loading Rate (SLR), Dry Solid (DS), the proportion of Volatile Solid to DS (VS/DS), and their association with the feeding and resting modes were extracted from the selected articles. The analysis was focused on the interconnections between operational parameters and system efficiency for Temperate type 1 (low intensity of solar radiation), Temperate type 2 (high intensity of solar radiation), and Tropical climates. Based on MA, we found the average SLRs of 50, 70, and 101 Kg.DM.m-2.year-1 for Temperate type 1, Temperate type 2, and Tropical climates respectively, and DS during the feeding of 33 %, 35 %, and 40 %. A qualitative comparison of Arid and Polar climates was also performed given the reduced number of studies available in these climates. The volume of the sludge reduced was 60 % higher and the height of accumulated sludge was annually 2 cm in the earthworm STRBs, and STEWs compared to typical STRBs, which was 6 cm annually in Tropical climates. Correlation analysis, media characterization, list of plant species, and the removal efficiency of STRBs in the residual sludge and leachate are mentioned as well.

Apocynin Attenuates Diabetes-Induced Skeletal Muscle Dysfunction by Mitigating ROS Generation and Boosting Antioxidant Defenses in Fast-Twitch and Slow-Twitch Muscles.

In response to diabetes mellitus, skeletal muscle is negatively affected, as is evident by reduced contractile force production, increased muscle fatigability, and increased levels of oxidative stress biomarkers. Apocynin is a widely used NADPH oxidase inhibitor, with antioxidant and anti-inflammatory potential. It has been effective for amelioration of a variety of disorders, including diabetic complications. Therefore, the present study was conducted to evaluate the effects and action mechanisms of apocynin in slow- and fast-twitch diabetic rat muscles. Male Wistar rats were rendered diabetic by applying intraperitoneally a single dose of streptozotocin (45 mg/kg). Apocynin treatment (3 mg/kg/day) was administered over 8 weeks. Fasting blood glucose (FBG), insulin tolerance and body weight gain were measured. Both slow (soleus) and fast (extensor digitorum longus, EDL) skeletal muscles were used for muscle function evaluation, oxidative stress markers, and evaluating gene expression using qRT-PCR. Treatment with apocynin significantly reduced FBG levels and enhanced insulin tolerance. Apocynin also prevented muscle contractile dysfunction in EDL muscle but had no significant effect on this parameter in soleus muscles. However, in both types of muscles, apocynin mitigated the oxidative stress by decreasing ROS levels and increasing total glutathione levels and redox state. Concomitantly, apocynin also statistically enhanced Nrf-2 and GLU4 mRNA expression and downregulated NOX2, NOX4, and NF-κB mRNA. Collectively, apocynin exhibits properties myoprotective in diabetic animals. These findings indicate that apocynin predominantly acts as an antioxidant in fast-twitch and slow-twitch muscles but has differential impact on contractile function.

Dual-pool, three-phase kinetic model of anaerobic digestion in batch mode.

The ability of anaerobic digestion to create value from waste gives it an important role in reducing greenhouse gas emissions and in the transition to a circular economy. For a better understanding of the digestion process and in order to reduce the number of time-consuming batch tests, an analytical model was developed to describe the kinetics of biogas production. Assuming that the organic fraction of the substrate has different degradation rates, the whole process was modelled as two groups of 1st order reactions. The model was tested with published data and showed an excellent performance in reproducing the experimental information. Moreover, its kinetic constants provided a useful insight into the internal processes of anaerobic digestion and the substrate characteristics. Given its accuracy in fitting the data, the model can be used as an auxiliary tool to determine the biogas potential, presenting itself as the most complete empirical model currently available.

Physico-chemical and electrostatic surface characterisation of mica mineral and its applicability on the adsorption of Safranin Orange and Reactive Black 5 dyes.

ABSTRACTThe combined approach of specific surface area (SSA), porosity, microprobe analysis (EMPA), transmission electron microscopy (TEM), scanning electron microscopy (SEM) equipped with EDX and infrared spectroscopy (FTIR) provided the mica mineral physico-chemical and morphological characterisation. The electrostatic surface properties were assessed through the determination of the Point of Zero Charge (pHPZC) by the drift method and the electrokinetic mica mineral features represented by the Isoelectric Point (pHIEP) which was carried out through zeta potential measurements. Adsorption tests were performed to correlate the surface charge behaviour of the mica mineral and its influence on the adsorption efficiency of two different dyes, namely: Safranin Orange (SO), as a cationic dye and Reactive Black 5 (RB5), as an anionic dye. The higher adsorption capacity SO dye was observed at pH 9 and achieved almost 83% of removal, while RB5 dye adsorption on mica surface had the highest result, about 45% of removal efficiency, on pH of 3. In both cases, the main mechanism identified that drove this results is the electrostatic force of attraction between the adsorbent edge surface charge (pH-dependent) and the ionic nature (anionic or cationic) of the pollutant dyes particles. The preliminary adsorption experiments demonstrated that the raw grounded mica mineral has a greater potential associated with its application on cationic dye removal in wastewater. The present study aimed to detail the main characteristics of the mica mineral in order to evaluate the potential use of such mineral residues in the removal efficiency of contaminated wastewater.

Enhanced biomethane production by co-digestion of mixed sewage sludge and dephenolised two-phase olive pomace.

In this study, co-digestion of mixed sewage sludge from a wastewater treatment plant (WWTP) and partially dephenolised two-phase olive pomace (DOP) as a co-substrate was addressed with the aim of improving the biodigestibility of both substrates. The introduction of DOP into WWTP anaerobic digester facilities could significantly increase biomethane production and enhance the sustainability of both activities. An improvement in the system's performance was supported by stability parameters: total alkalinity increased and stabilised with the addition of 5% v/v DOP, and the specific energy loading rate was maintained at 0.177 ± 0.03 d-1, which indicated better buffer capacity and stability in the bioreactor, and the possibility of enhancing the organic loading rate. In terms of average daily biogas production rate, an increase of 39% was achieved, up to 0.39 ± 0.11 L L-1d-1. Moreover, there was a 40% and 37% improvement in specific methane production and methane production rate, respectively, up to 0.28 ± 0.02 L CH4 g TVS-1 and 0.26 ± 0.08 L L-1d-1. In addition, the proposed strategy leads to an energy saving of 20,328.6 kWh year-1 at the WWTP as a result of the electric energy production surplus, corresponding to an annual saving of €3293.23.

Diagnóstico prenatal del retraso de crecimiento intrauterino, en pacientes asistidas en el Hospital Regional de Ciudad del Este, año 2018

Resumen La restricción de crecimiento intrauterino es un problema de salud que aumenta su conocimiento en la población puede generar acciones en la salud pública, asimismo se asocia a mayor riesgo en adolescentes, a bajo nivel de instrucción, población marginal, bajo peso de la madre y de atención prenatal inadecuado, el objetivo de este trabajo fue la de analizar el diagnóstico prenatal del retraso de crecimiento intrauterino mediante la ecografía, en pacientes asistidas en el Hospital Regional de Ciudad del Este, año 2018, se realizó un estudio observacional descriptivo retrospectivo, la muestra de estudio estuvo constituida por 73 historia clínica de madres que presentaron restricción de crecimiento intrauterina diagnosticada por ecografía se consignaron en un formato de recolección de datos a través de la revisión de historias clínicas, se organizaron en una base de datos en Excel y se realizó un análisis descriptivo. El 41% de las madres presentaron edades entre 19 a 34 años, se observó mayor índice con el 64 con procedencia de zonas rurales respecto a las madres con procedencias de zonas urbanas, las mayorías de las madres presentaron un nivel secundario concluida, el 52% de las madres presentaron controles prenatales entre 0 a 5 controles, las madres con edad gestacional < 37 semanas tuvieron significativamente (p=0.000) mayor frecuencia recién nacido con retardo de crecimiento intrauterino, el 29% de las madres que tuvieron bajo peso presentaron recién nacidos con retardo de crecimiento intrauterino, 21 de las madres nulíparas presentaron recién nacidos con retardo de crecimiento intrauterino y el 44% de las madres con intervalo intergenésico menor de 2 años presentaron recién nacidos con retardo de crecimiento intrauterino, y el mayor índice de hijos con retardo de crecimientos intrauterina presentaron madres con una ganancia entre 6 a 9 Kg y se concluye que el retardo de crecimiento intrauterino tiene asociación con factores sociodemográficos y obstétricos, que algunos de ellos pueden ser intervenidos.

Abstract The restriction of intrauterine growth is a health problem that increases their knowledge in the population can generate actions in public health, it is also associated with increased risk in adolescents, at a low level of education, marginal population, low mother and child weight inadequate prenatal care, the objective of this work was to analyze the prenatal diagnosis of intrauterine growth retardation by ultrasound, in patients assisted in the Regional Hospital of Ciudad del Este, year 2018, a retrospective descriptive observational study was performed, the sample The study consisted of 73 clinical records of mothers who presented intrauterine growth restriction diagnosed by ultrasound were recorded in a data collection format through the review of medical records, they were organized in a database in Excel and a descriptive analysis showing the following results 41% of the madr es presented ages between 19 and 34 years old, a higher index was observed with 64 originating from rural areas with respect to mothers from urban areas, most of the mothers presented a completed secondary level, 52% of the mothers presented controls Prenatal between 0 and 5 controls, mothers with gestational age <37 weeks had significantly (p = 0.000) higher frequency newborn with intrauterine growth retardation, 29% of mothers who were underweight presented newborns with intrauterine growth retardation , 21 of the nulliparous mothers presented newborns with intrauterine growth retardation and 44% of the mothers with intergenic interval less than 2 years presented newborns with intrauterine growth retardation, and the highest rate of children with intrauterine growth retardation presented mothers with a gain between 6 to 9 Kg and it is concluded that the intrauterine growth retardation tie Ne association with sociodemographic and obstetric factors, which some of them can be intervened.

Integration of Membrane Processes for By-Product Valorization to Improve the Eco-Efficiency of Small/Medium Size Cheese Dairy Plants.

Goat and second cheese whey from sheep's milk are by-products of the manufacture of goat cheeses and whey cheeses from sheep. Due to their composition which, apart from water-about 92%-includes lactose, proteins, fat, and minerals, and the elevated volumes generated, these by-products constitute one of the main problems facing to cheese producers. Aiming to add value to those by-products, this study evaluates the efficiency of ultrafiltration/diafiltration (UF/DF) for the recovery of protein fraction, the most valuable component. For a daily production of 3500 and using the experimental results obtained in the UF/DF tests, a membrane installation was designed for valorization of protein fraction, which currently have no commercial value. A Cost-Benefit Analysis (CBA) and Sensitivity Analysis (SA) were performed to evaluate the profitability of installing that membrane unit to produce three new innovative products from the liquid whey protein concentrates (LWPC), namely food gels, protein concentrates in powder and whey cheeses with probiotics. It was possible to obtain LWPC of around 80% and 64% of crude protein, from second sheep cheese whey and goat cheese whey, respectively. From a survey of commercial values for the intended applications, the results of CBA and SA show that this system is economically viable in small/medium sized cheese dairies.

Valorization of Goat Cheese Whey through an Integrated Process of Ultrafiltration and Nanofiltration.

Goat cheese whey is a co-product that comes from goat cheese manufacture. Due to its high organic load, adequate treatment is necessary before its disposal. Additionally, the recent growing interest in caprine products, attributed to their specific nutritional and nutraceutical characteristics, such as the lower allergenicity of their proteins and higher content of oligosaccharides, compared with bovine products, made the recovery of goat cheese whey a challenge. In this study, an integrated process for the recovery of sweet goat whey components was carried out. It includes filtration, centrifugation and pasteurization, followed by sequential membrane processes, ultrafiltration/dilution, nanofiltration of ultrafiltration permeates in dilution mode and the concentration/dilution of nanofiltration retentates. Ultrafiltration was performed with membranes of 10 and 1 kDa. Membranes of 10 kDa have higher permeate fluxes and, in a single stage of dilution, allowed for better protein retention and higher lactose purity, with a separation factor of 14. The concentration of lactose by nanofiltration/dilution led to the retention of almost all the lactose in retentates and to a final permeate, whose application in cheese dairy plants will allow for the total recovery of whey. The application of this integrated process in small- or medium-sized goat cheese dairies can represent an important contribution to their sustainability.